A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
نویسندگان
چکیده
BACKGROUND Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. RESULTS We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. CONCLUSION pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.
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Genome structure in Streptomyces spp.: adjacent genes on the S. coelicolor A3(2) linkage map have cotransducible analogs in S. venezuelae.
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